EPA STAR Grant Abstract 1. Research Category and Sorting Code: 99-NCERQA-E1 (Ecological Indicators) 2. Title: Multi-level Indicators of Ecosystem Integrity in Alpine Lakes of the Sierra Nevada

A. Objectives : The overall objective of the proposed research is to develop protocols for environmental assessments of alpine lakes in the Sierra Nevada with a range of human impacts. These assessments will be conducted over the range of levels of biological organization (molecular to ecosystem) utilizing currently available assessment techniques and with the addition of two new ecological indicators. The use of population genetics analysis as a response indicator and the use of molecular biomarkers of exposure to contaminants as a diagnostic indicator are proposed for incorporation into monitoring and assessment programs for surface waters. These indicators will provide critical information concerning the status of population diversity and stability and concerning the exposure to non-persistent, non-bioaccumulative contaminants. This is information that is missing from current monitoring and assessment protocols.